Reporter

Part:BBa_K945004:Design

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-26)


Pichia pastoris optimized His-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 1315
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
    Illegal BamHI site found at 1286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 1315
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 1315
    Illegal AgeI site found at 1005
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 877
    Illegal BsaI.rc site found at 1269


Design Notes

The codon optimization utilized had to take in account the illegal restriction sites to be avoided in order for it to be standardized.


Source

The sequence, previous to its modification, came from the optimization of the wild-type GFP found in Aequorea victoria.

References